Detection of cerebral NAD + by in vivo 1 H NMR spectroscopy
Robin A. de Graaf 1 and Kevin L. Behar 1
Yale University, New Haven, CT, United
Nicotinamide adenine dinucleotide (NAD+) has a central
role in cellular metabolism and energy production and is
related to gene expression, calcium mobilization, aging,
cell death and timing of metabolism via the circadian
rhythm. The in vivo detection of NAD+ has been limited
and has only recently become feasible with high-field
31P NMR spectroscopy. Here 1H NMR spectroscopy is
presented as a simple, but robust alternative for the in
vivo detection of NAD+. Special attention is given to
the interaction between NAD+ and water magnetization.
Perturbation of the water protons should be minimized to
ensure full NAD+ signal detection efficiency.
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