Meeting Banner
Abstract #2809

Monitoring re-cellularization of acellularized organs with iron oxide nanoparticles and T2W-MRI

Joseph E Kobes 1 , George I Georgiev 2 , Anthony V Lewis 3 , Horacio L Rilo 3 , Zain Khalpey 3 , and Mark D Pagel 4

1 Biomedical Engineering, University of Arizona, Tucson, AZ, United States, 2 Department of Surgery, University of Arizona, Tucson, Arizona, United States, 3 Department of Surgery, University of Arizona, Tucson, AZ, United States, 4 Department of Biomedical Engineering, University of Arizona, Tucson, Arizona, United States

Re-seeding organ scaffolds with a patients own cells is a promising approach to improve organ transplant procedures. This study investigated whether T2-weighted MRI can track the reseeding of murine liver scaffolds. Liver cells were labeled with iron oxide nanoparticles that were coated with polystyrene, fluorophore, protamine sulfate, and glutamine. An acellularized mouse liver was re-perfused with the iron-labeled cells. T2-weighted MRI monitored initial sparsely populated cell loading, and heavily populated cell load after 8 days of cell growth, which was confirmed with confocal microscopy and SEM of liver biopsies. These findings demonstrate that MRI can track re-cellularization of acellularized organs.

This abstract and the presentation materials are available to members only; a login is required.

Join Here