Abstract #2934
Metabolic reprograming in IDH mutant glioma cells
Jose Luis Izquierdo Garcia 1 , Pia Eriksson 1 , Myriam Chaumeil 1 , Russell O Pieper 2 , Joanna J Phillips 2 , and Sabrina M Ronen 1
1
Radiology, UCSF, San Francisco, CA, United
States,
2
Neurological
Surgery, Helen Diller Research Center, UCSF, San
Francisco, CA, United States
This study demostrates that the activity of mutant IDH1
results not only in production of the oncometabolite
2-HG, but also in a wider metabolic reprogramming. Two
cell models were investigated by 1H-MRS: the U87-based
and the E6/E7/hTERT Normal Human Astrocyte (NHA)-based
models. Metabolic profiling discriminated between
wild-type and mutant IDH cells. A significant drop in
the concentration of acetate, aspartate, glutamine and
myo-inositol was observed in U87IDHmut cells and lower
creatine and lactate concentration was observed in
NHAIDHmut cells. In both models, IDHmut cells showed a
lower concentration of glutamate and phosphocholine as
well as the expected elevation in 2-HG
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