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Abstract #3364

Quantification of 2HG Metabolite in Human Glioma Tissues by NMR and Mass Spectrometry

Hyeon-Man Baek 1,2 , Yun-Ju Lee 1 , Gregory Hyung Jin Park 1 , Eun-Hee Kim 1 , and Chaejoon Cheong 1

1 Division of MR Research, Korea Basic Science Institute, Ochang, Chungbuk, Korea, 2 Department of Bio-Analytical Science, University of Science & Technology, Yuseong-gu, Daejeon, Korea

Accurate measurement of 2HG levels by them is challenging due to significant overlap with neighboring metabolites such as GABA, Glu, Gln, and m-Ins. In addition, these techniques have limitations to detect and quantify the 2HG levels from wild type IDH tumors because of the sensitivity issues. In this study, in vivo 1H-MRS, and in vitro high resolution 1H-NMR, and Mass spectroscopy (MS) have been applied to the quantification of metabolites in wild type and mutant IDH1/2 brain tumor extracts. A significant increase in the levels of 2HG was observed in the IDH1/2 mutants, which is consistent with the previous published results

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