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Abstract #3752

Diffusion-weighted HR-MAS of biopsies to obtain separated fat-free metabolite and lipid spectra. A feasibility study.

Galle Diserens 1 , Martina Vermathen 2 , Christina Stahl 3 , Nicholas T. Broskey 4 , Chris Boesch 1 , Francesca Amati 1,4 , and Peter Vermathen 1

1 Depts Clinical Research and Radiology, University Bern, Bern, Bern, Switzerland, 2 Dept. of Chemistry & Biochemistry, University Bern, Bern, Switzerland, 3 Dept. of Clinical Veterinary Medicine, Clinical Radiology, Vetsuisse-Faculty, University Bern, Bern, Switzerland, 4 Dept. of Physiology, University of Lausanne, Lausanne, Switzerland

HR-MAS NMR diffusion measurements with strong and low diffusion weighting were used to obtain fat-free spectra: Pure lipid spectra obtained at strong diffusion weighting were subtracted from those obtained at low diffusion weighting, which include both, small metabolites and lipids. This operation results in a spectrum with low lipid contamination under specific metabolite peaks, allowing for a more accurate quantification. This technique has therefore potential to improve chemometric analysis for biomarkers detection. Moreover, the diffusion scans permit a separate lipid analysis and a regular diffusion analysis without expanding scan time.

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