Combination of a 13 C cryoprobe with hyperpolarization allows real time observation of pyruvate carboxylation in the perfused mouse heart
Colin Purmal 1 , Blanka Kucejova 1 , Shawn Burgess 1 , Craig Malloy 1 , Dean Sherry 1 , and Matthew E Merritt 1
AIRC, UTSW Medical Center, Dallas, TX,
Murine models of myocardial metabolism are a pervasive
tool used by the cardiovascular research community.
Development of methods for monitoring energy metabolism
in the perfused mouse heart would augment the
understanding of a variety of myocardial pathologies and
dysfunctions. Here, hyperpolarized pyruvate is combined
optimized cryogenic probe to produce an approximate
sensitivity gain of 140,000x for carbon spectroscopy.
The resulting spectra in the functioning heart allow
pyruvate carboxylation to be monitored in real time, a
pathway accepted to have a relative activity of about 5
% of that of pyruvate dehydrogenase.
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