Validation of orientation distribution functions in 3D using confocal microscopy
Kurt Schilling 1 , Yurui Gao 1 , Vaibhav Janve 1 , Iwona Stepniewska 2 , Prasanna Parvathaneni 3 , Hua Li 1 , Bennett A Landman 4 , and Adam W Anderson 1
VUIIS, Vanderbilt University, Nashville, TN,
Vanderbilt University, Nasvhille, United States,
Engineering, Vanderbilt University, TN, United States,
Engineering, Vanderbilt University, Nashville, TN,
Validation of fiber orientation and anisotropy estimates
from diffusion MRI (dMRI) studies has so far been
limited to 2D analysis of histological sections. Here,
we use structure tensor analysis to extract the 3D
histological fiber orientation distribution (FOD) from
confocal z-stacks, which then serves as a gold standard
for comparisons with various dMRI acquisition protocols
and signal models. Our results show good agreement
between histological and dMRI estimates of fiber
orientation in voxels containing nearly parallel fibers.
Further, we find that Q-ball and DSI analysis are also
able to resolve crossing fibers with similar accuracy.
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