13C NMR studies of lymphoma and melanoma cells in the perfusion bioreactor and in vivo xenografts for flux calculation
Seung-Cheol Lee 1 , Jeffrey Roman 1 , Kavindra Nath 1 , David Nelson 1 , Kevin Muriuki 1 , Alexander Shestov 1 , and Jerry Glickson 1
Department of Radiology, University of
Pennsylvania, Philadelphia, PA, United States
Time course 13C NMR study was performed in the perfused
lymphoma and melanoma cells as well as in vivo
xenografts for detailed metabolic flux calculation. mTOR
signaling inhibitor rapamycin was administered to
lymphoma cells and xenografts. Well resolved time course
13C NMR spectra were obtained from both perfused cells
and in vivo tumors. mTOR signaling inhibition decreased
fluxes to lactate, glutamate as well as glycogen.
Melanoma xenografts exhibited higher TCA cycle flux than
lymphoma xenografts. Quantitative flux calculation is
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