Abstract #1917
A Novel Assay for the in vivo Detection of Reactive Oxygen Species Using MRI
Gary Stinnett 1 , Kelly Ann Moore 1 , Errol Loc Samuel 2 , Ming Ge 3 , Brett Graham 3 , James Tour 2 , and Robia G Pautler 1
1
Molecular Physiology and Biophysics, Baylor
College of Medicine, Houston, Texas, United States,
2
Department
of Chemistry, Rice University, Houston, Texas, United
States,
3
Molecular and Human Genetics, Baylor
College of Medicine, Houston, Texas, United States
Due to their paramagnetic properties, increased
production of superoxide and hydroxyl radicals can cause
decreases in T1 relaxation. Animals with increased
superoxide and hydroxyl radical production will have
lower T1 values in the liver compared to wild type
controls. To determine that T1 reduction is due
specifically to increased superoxide and hydroxyl
radicals, animals were then treated with superoxide
scavenging PEG-HCCs. These particles rendered the
superoxide and hydroxyl anions inert, increasing T1
values to that of wild type controls. We therefore
demonstrate that it is possible to use MRI in
conjunction with PEG-HCCs to measure in vivo ROS levels.
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