NanoIron Phantom to Validate In-Vivo Iron Mapping
Stephen E. Russek 1 , Kathryn E. Keenan 1 , Karl Stupic 1 , Michael A. Boss 1 , Zydrunas Gimbutas 1 , Andrew M. Dienstfrey 1 , and Robert J. Usselman 2
NIST, Boulder, CO, United States,
of Montana, Bozeman, MT, United States
We investigated several materials for use in a nano-iron
phantom including Fe chelates, hemoglobin, recombinant
human ferritin, horse spleen ferritin (HSF), Feraheme,
Molday ion, nanoComposix iron oxide, textured (chained)
nanoparticles. The range of concentrations was selected
to match brain iron concentrations in healthy and
diseased tissue (100-200 ppm). A key advance was to
develop recombinant human ferritin by obtaining H-chain
DNA sequences, amplifying with PCR, splicing them into
pET30a(+) plasmids, and transfecting E. Coli. Various
mineralization techniques were applied to mimic pure
phase, healthy, and pathologic forms of human ferritin.
These materials were incorporated into phantoms and
characterized using multiparameter mapping to assess
ability to measure concentration and form in-vivo.
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