Lipid characterization of different organs using HR-MAS NMR spinning speed variation.
Galle Diserens 1 , Christina Precht 2 , Martina Vermathen 3 , Anna Oevermann 4 , Chris Boesch 1 , and Peter Vermathen 1
Depts. Radiology and Clinical Research,
University Bern, Bern, Switzerland,
of Clinical Veterinary Medicine, University Bern, Bern,
of Chemistry & Biochemistry, University Bern, Bern,
of Clinical Research and Veterinary Public Health,
University Bern, Bern, Switzerland
Proton HR-MAS NMR can assess mobile lipids from
different tissues. The study purpose was to better
understand the origin of visible lipids and the cause of
this lipid visibility, by investigating the effect of
spinning speed variation in HR-MAS NMR I) in different
organs, comprising liver, cardiac muscle, breast, kidney
and adipose tissue, and II) in white and grey matter
accompanied by histological lipid visualization. The
spinning speed significantly affects the lipid
visibility. Histology results support the hypothesis
that lipids from myelinated membranes are NMR-visible at
high spinning speed, while lipid droplets may be less
affected by the spinning speed.
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