A reproducible experimental protocol for longitudinal rat fMRI studies: electrical mystacial pad stimulation under isoflurane anesthesia
Shin-Lei Peng 1,2 , Ling-Yi Huang 1 , Sheng-Min Huang 1 , Yi-Chun Wu 3 , Hanzhang Lu 2 , Fu-Chan Wei 4 , Chih-Jen Wen 4 , Hui-Yu Cheng 4 , Chih-Hung Lin 4 , and Fu-Nien Wang 1
Department of Biomedical Engineering and
Environmental Sciences, National Tsing Hua University,
Imaging Research Center, University of Texas
Southwestern Medical Center, Dallas, Texas, United
Imaging Center, Chang Gung Memorial Hospital, Taoyuan,
of Plastic and Reconstructive Surgery, Chang Gung
Memorial Hospital, Taoyuan, Taiwan
fMRI plays an role in neuroscience researches.
Investigating animal fMRI studies is an essential step
toward understandings of cortical reorganization after
surgery. Most researches use alpha-chloralose as an
anesthetic, which is improper for follow-up studies.
Here, we established a new protocol of rat electric
mystacial pad (MP) stimulation under isoflurane
anesthesia with advantages of increased BOLD sensitivity
and possibility for longitudinal studies. Results show
MP stimulation exhibited reproducible activations in the
sometosensory cortex. In terms of the extension of
activations and induced BOLD signal changes, results
from MP stimulation under isoflurane anesthesia are
comparable with those under alpha-chloralose anesthesia.
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