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Abstract #0673

Detection of inflammatory cell function using 13C MRS of hyperpolarized 13C-labeled arginine

Chloe Najac1, Myriam M Chaumeil1, Gary Kohanbash2, Caroline Guglielmetti3, Jeremy Gordon1, Hideho Okada2, and Sabrina M Ronen1

1Radiology and Biomedical Imaging, University of California San Francisco, San Francisco, CA, United States, 2Neurological Surgery, University of California San Francisco, San Francisco, CA, United States, 3Bio-Imaging Lab, University of Antwerp, Antwerpen, Belgium

Myeloid-derived suppressor cells (MDSCs) are inflammatory cells in the tumor microenvironment that inhibit T-cell-mediated immunosuppression by expressing high levels of arginase. Arginase catalyzes the breakdown of arginine into urea. To monitor the enzymatic conversion, we developed a new hyperpolarized (HP) probe, namely [guanido-13C]-arginine. We first characterized the probe and confirmed the production of HP 13C urea in solution with different arginase concentrations. Then, we demonstrated its potential to probe the increase in arginase activity in MDSCs. This new HP probe could serve as a readout of MDSC function in tumor and its inhibition following MDSC-targeted immunotherapies.

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