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Abstract #0761

Validation and Optimization of Calibrated fMRI from oxygen-sensitive Two-Photon Microscopy of the mouse brain

Louis Gagnon1,2,3, Sava Sakadzic4, Frederic Lesage2, Philippe Pouliot2, Anders M Dale5, Anna Devor5, Richard B Buxton5, and David A Boas3

1Department of Medicine, Laval University, Quebec, QC, Canada, 2Department of Electrical Engineering, Ecole Polytechnique Montreal, Montreal, QC, Canada, 3Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Charlestown, MA, United States, 4Athinoula A. Martinos Center for Biomedical Imaging, Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Chalestown, MA, United States, 5Department of Radiology and Neuroscience, UCSD, La Jolla, CA, United States

Calibrated fMRI allows to estimate relative changes in the Cerebral Metabolic Rate of Oxygen Consumption (rCMRO2) from combined BOLD and ASL measurements during a functional task. Here, we improved the accuracy of the approach by using Two-Photon microscopic measurements of the cortical microvasculature together with first principle Monte Carlo simulations of proton diffusion across the two-photon volumes. Our method allowed (1) to validate Calibrated fMRI from the microscopic point of view and (2) to optimize the free parameters of the biophysical model assumed, therefore increasing the accuracy of this method to estimate rCMRO2.

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