Tracking transplanted cells with paramagnetic fluorinated nanoemulsions
Alexander A. Kislukhin1, Hongyan Xu1, Stephen R. Adams2, Kazim H. Narsinh1, Roger Y. Tsien2,3, and Eric T. Ahrens1
1Radiology, University of California San Diego, La Jolla, CA, United States, 2Chemistry & Biochemistry, University of California San Diego, La Jolla, CA, United States, 3Howard Hughes Medical Institute, La Jolla, CA, United States
Fluorine-19 magnetic resonance imaging (MRI) probes are used to label cells for quantitative in vivo tracking of cell therapies and visualizing inflammation. To reduce the 19F spin-lattice relaxation time (T1) and enable rapid imaging and improved cell detection sensitivity, we prepared metal-binding fluorinated nanoemulsions, and then metalated them with a panel of transition and lanthanide ions. Iron(III) tris-β-diketonate PFPE nanoemulsion was observed to have superior MRI properties (19F T1 as low as 6 ms). Overall, these agents can yield a multifold improvement in detection sensitivity over previously employed 19F MRI methods to track transplanted cells.
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