Renal blood flow is thought to be reduced in subjects of diabetic nephropathy (DN). However, there is limited amount of quantitative data on renal blood flow in patients with DN. In this study, ASL MRI data was acquired in 28 patients with diabetes and stage-3 CKD along with 30 healthy controls. Renal blood flow was found to be significantly lower in subjects with DN with a large Cohen’s d value. Renal blood flow also showed a significant correlation with eGFR and age was not found to be a significant confounder in this relationship.