Intraperitoneal substrate administration for ¹³C metabolic imaging in a mouse model of abdominal metastasis
Justin Y.C. Lau1,2, Aws Abdul-Wahid3, Albert P. Chen4, Jean Gariépy1,3, and Charles H. Cunningham1,2
1Medical Biophysics, University of Toronto, Toronto, ON, Canada, 2Physical Sciences, Sunnybrook Research Institute, Toronto, ON, Canada, 3Biological Sciences, Sunnybrook Research Institute, Toronto, ON, Canada, 4GE Healthcare, Toronto, ON, Canada
Conventionally, hyperpolarized 13C substrates are administered via intravenous injection. In this abstract, a novel route of hyperpolarized substrate delivery via intraperitoneal injection is demonstrated for observing metabolism in a mouse model of abdominal metastasis. 2D CSI revealed lactate signal in tumour-bearing mice, but only pyruvate signal in a control mouse. An extended time window of dynamic metabolic imaging may be possible with intraperitoneal administration due to the longer in vivo pyruvate T1 of 54 s as measured by dynamic 3D EPI. Intraperitoneal administration of hyperpolarized 13C substrates is a promising complementary technique well suited for observing poorly vascularized metastatic nodules.
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