Abstract #2337

Concentration-dependent hepatic metabolism in vivo using a near physiological dose range of hyperpolarized [1-13C]pyruvate

Emine Can¹, Jessica A. M. Bastiaansen^2,3, Hikari A. I. Yoshihara^4,5, Rolf Gruetter^3,5,6, and Arnaud Comment¹

¹Institute of Physics of Biological Systems, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland, ²Department of Radiology, University Hospital Lausanne (CHUV), Lausanne, Switzerland, ³Department of Radiology, University of Lausanne (UNIL), Lausanne, Switzerland, ⁴Institute of Physics of Biological Systems, EPFL, Lausanne, Switzerland, ⁵Laboratory for Functional and Metabolic Imaging, EPFL, Lausanne, Switzerland, ⁶Department of Radiology, University of Geneva, Geneva, Switzerland

Hyperpolarized ¹³C-labeled pyruvate provides assessment of real-time liver mitochondrial enzymatic activities directly by labeling TCA cycle intermediates. However the technique is limited by the requirement of supraphysiological concentrations due to the low basal concentrations of metabolic intermediates. In this study we showed the feasibility of detecting liver metabolism in vivo with HP ¹³C pyruvate administered at plasma concentrations of at most 7-fold of the basal levels. Different metabolic response to the concentration change shows that the adaptation to supraphysiological levels can obscure feeding state-depending metabolic differences in liver.

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