Per Mose Nielsen1, Christoffer Laustsen1, Haiyun Qi1, Thomas Nørlinger1, Emmeli Mikkelsen1, Rikke Nørregaard1, and Hans Stødkilde Jørgensen1
Renal
I/R-I is a leading cause of AKI in several disease states; there is a current
lack of precise methods to directly assess cortical tubular injury. In the
present study, we investigated the in
situ alterations of metabolic conversion of pyruvate to lactate in a
unilateral I/R-I rat model using [1-13C]pyruvate magnetic resonance. A significantly
reduced lactate-to-pyruvate ratio of 25% as compared to control was found in
the I/R-I kidney, concomitant with a reduced LDH activity, which was specific
for the I/R-I kidney. The lowered lactate-to-pyruvate ratio and LDH activity
strongly correlated with general tissue damage.
