Abstract #4341

2HG Optimized PRESS MRS for Metabolic Profiling of IDH-mutant Gliomas in Patient-Derived Orthotropic Mouse Model of Human Brain Tumor

Vivek Tiwari¹, Tomoyuki Mashimo², Sandeep Kumar Ganji¹, Zhongxu An¹, Keith Hulsey³, Vamsidhara Vemireddy², Shanrong Zhang¹, Elizabeth Maher², Robert Bachoo², and Changho Choi¹

¹Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, TX, United States, ²Department of Internal Medicine, UT Southwestern Medical Center, Dallas, TX, United States, ³Department of Radiology, UT Southwestern Medical Center, Dallas, TX, United States

2-Hydroxyglutarate (2HG) is elevated in gliomas harboring IDH1 or IDH2 mutations, and can be a biomarker for the diagnosis of IDH-mutant gliomas. The present study was undertaken to develop PRESS MRS Methodology for 2HG-detection with enhanced precision, and studying 2HG kinetics together with metabolic profiling in Patient-Derived Xenograft Orthotropic Mouse model. In-vivo PRESS MRS was performed at an optimized long-TE of 96ms. A large inverted-signal of 2HG at 2.25-ppm was obtained from IDH-mutant gliomas, well separated from neighboring signals of positive-polarity, with minimal interference from macromolecules. The method was successfully employed to detect 2HG and brain metabolism with tumor progression.

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