Currently, various 3D MR sequences such as 3D FSE, GRE, and steady-state sequences have been developed and used to obtain MR images of submillimeter thicknesses in the spine. However, the question,“which 3D sequence has a better image contrast of the tissues in spinal MRI?”, has come up. Therefore, we examined optimal imaging parameters of variable refocus flip angle 3D FSE (Cube) and 3D Fiesta-c sequences for T2-weighted images in cervical spine MRI and compared the two different 3D sequences for better image contrasts of the tissues on T2-weighted images in cervical spine MRI. In conclusion, 3D FSE Cube T2-weighted images has excellent image contrasts between the spinal cord versus other tissues except cerebrospinal fluid, vertebral body, and subcutaneous fat in the cervical spine compared to 3D Fiesta-c T2-weighted images. Therefore, 3D FSE Cube sequence is believed to be more appropriate for T2-weighted images in cervical spine MRI compared to 3D Fiesta-c sequence.