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Abstract #0280

Validation of the two-pool diffusion model in post-mortem white matter using the CLARITY method

Jakob Georgi1, Markus Morawski2, Carsten Jäger1,2, and Harald E. Möller1

1Max-Planck-Institute for Human Cognitive and Brain Sciences, Leipzig, Germany, 2Paul-Flechsig-Institute for Brain Research, Leipzig, Germany

Water diffusion in tissues is known to be non-Gaussian. Moreover, two different water regimes have been found in brain tissue and assigned to a bulk-like compartment and water in contact with macromolecules. Here, we investigate the influence of membranes in post-mortem white matter, which are assumed to be responsible for the second pool observed in MR-diffusion measurements. Using a newly developed CLARITY method, which removes lipids from brain tissue while keeping the brain structure intact, we found that the slow compartment vanished while the mobility of the fast pool increased, which directly demonstrates the influence of membranes on water dynamics.

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