DCE-MRI is already a standard approach to evaluating tumor perfusion in NPC, but it may induce severe side-effects. ASL is a noninvasive MRI technique that has been mainly used to achieve perfusion imaging in central nerve system. In this study, we attempt to evaluate the application of ASL in NPC. Thirty-eight newly diagnosed NPC patients underwent 3D ASL and DCE-MRI perfusion scans on a 3.0-T MRI system. ASL BF value and DCE-MRI parameters were calculated and compared. Statistically significant correlation was found between them. Therefore, 3D ASL may provide a potential alternative to DCE-MRI in NPC diagnosis and therapy evaluation.