Abstract #0546

Validation of Cardiac Diffusion Tensor MRI using Transparent Tissue Preparation (CLARITY) with 3D Optical Microscopy

Christopher Nguyen¹, Sang-Eun Lee², Jongjin Yoon³, Hyuk-Jae Chang², Sekeun Kim², Chul Hoon Kim³, and Debiao Li^1,4

¹Biomedical Imaging Research Institute, Cedars-Sinai Medical Center, Los Angeles, CA, United States, ²Division of Cardiology, Yonsei University College of Medicine, Seoul, Korea, Republic of, ³Departement of Pharmacology, Yonsei University College of Medicine, Seoul, Korea, Republic of, ⁴Bioengineering, University of California Los Angeles, Los Angeles, CA, United States

The myocardium consists of a complex 3-dimensional (3D) microstructure has been shown to be perturbed in the presence of myocardial ischemia. Recently, diffusion tensor magnetic resonance imaging (DT-CMR) was introduced which can characterize the 3D tissue microstructure in intact myocardium. However, past histologic validation of DTI has been limited since traditional pathology allows only 2D optical microscopy after potentially destructive tissue sectioning. We present a novel approach to validate the derivation of the myocardial fiber orientation (MFO) using DT-CMR with 3D histology using a non-destructive, transparent-tissue preparation technique (CLARITY). Results indicate MFO derived from 3D histology and DT-CMR are strongly concordant.

This abstract and the presentation materials are available to members only; a login is required.

Join Here