Adenosine monophosphate-activated protein kinase (AMPK) is an evolutionarily conserved signaling molecule essential for cellular energy balance. AMPK senses metabolic stress and integrates diverse physiological signals to restore energy balance. Its role in the normal brain development is not well understood. The purpose of this study was to assess whether neurochemical profiles of developing mouse brain are affected by knocking out of AMPK enzyme in a region-specific manner. Observed changes in metabolite levels (Lac, Glu) indicate reduced energy metabolism in AMPK knockout mice relative to WT controls. In addition, changes in myo-Ins suggest osmotic stress.