Tractography-based parcellation of the Nucleus Accumbens (NAc) was performed using high-resolution diffusion data from the Human Connectome Project. Analysis of clustering indicated the NAc was best separated into 2 subregions, consistent with anatomical and histological studies of animals. Output of the procedure gave qualitatively similar results across subjects, producing clusters that tended to occupy the ventromedial and dorsolateral portions of the NAc. The ventromedial subregion demonstrated increased task fMRI activation and displayed preferential connections to known biased projection areas such as medial orbitofrontal cortex. Finally, qualitatively similar results were obtained by performing the clustering procedure on a separate, lower-quality dataset.