The aim of this study was to compare two AIFs derived from DCE (AIFDCE) and DSC MR imaging (AIFDSC) in terms of the diagnostic accuracy and reliability of pharmacokinetic parameters from DCE MRI for differentiation of high grade from low grade glioma. This retrospective study included 70 patients with pathologically confirmed gliomas. In all of the patients, we performed preoperative DSC and DCE MRI, and two AIFs (AIFDSC and AIFDCE) were obtained from each image. Pharmacokinetic parameters (Ktrans, Vp, and Ve) were processed. DCE MRI parameters obtained using AIFDSC showed better accuracy and reliability than those derived from AIFDCE.