31 patients with high grade glioma were recruited at both pre- and post-radiation therapy. 3D EPSI was employed on a Siemens 1.5 T scanner. NAA and Cho in lesion are normalized to Cr at contralateral part. At pre-radiation, Cho/Cr at lesion is significantly higher than that in contralateral part (p = 0.003). From pre- to post-radiation, Cho/Cr at lesion is significantly decreased (p < 0.007). Elevated Cho level can be an indicator for residual glioma tumor after surgery. Cho/Cr ratio can be used to monitor the treatment response. EPSI technique can be a promising way to monitor treatment response.