The human tongue is known to have a complex architecture of muscles. To fully understand how the muscle fibers are connected and how their relative position could affect the tongue functionality, diffusion weighted imaging is needed. Diffusion Spectrum Imaging (DSI) is able to characterize the fiber structure on a sub-voxel level including the fiber crossing or branching. As DSI sequences are usually time-consuming, in-vivo studies using DSI can be challenging. In this study, we present for the first time DSI of the post-mortem human tongue. and the associated tractography delineating the tongue muscle fibers. DSI has the ability to identify fiber crossings within the human tongue.