Nicotinamide adenine dinucleuotide (NAD+) is a ubiquitous molecule present in all cells and tissues of the body with an important role in the redox reactions and metabolism. Small changes in NAD+ levels may lead to oxidative stress and may be a cause for various disorders. Currently, NAD+ can be detected in vivo only by 31P NMR spectroscopy. Recently, NAD+ measurement with 1H MRS in the human brain with a surface coil was demonstrated. In the present study, we show a novel MR pulse sequence for the in vivo, single voxel localized detection of NAD+ from the human brain at 7T.