The purpose of this study was to characterize changes in hyperpolarized 13C-pyruvate spectra in the liver and kidneys of two contrasting models of diabetes, obese Zucker diabetic fatty (ZDF) rats and streptozotocin(STZ)-treated (insulin-deficient) wild type Zucker rats. The results were interpreted in combination with transcriptional analysis of freeze-clamped tissue samples from these animals. Hyperpolarized lactate levels were elevated in both models while hyperpolarized alanine signals clearly diverged, decreasing in the type 1 model but increasing in type 2. Overall, the results suggest a complex interplay of transcriptional and substrate-level effects in determining the metabolic phenotype in diabetes.
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