We evaluated the reliability and clinical value of amide proton transfer-weighted (APTW) MR imaging at 3 Tesla in adult gliomas. Fifty-seven patients with primary gliomas were recruited and scanned. Two radiologists evaluated the location and size of the APTW hyperintensity and enhancing areas, and measured the tumor and contralateral normal-appearing white matter (CNAWM) APTW values. The correlation between relative APTW (rAPTW) values and pathologic grades was calculated. Results showed APTW analysis had good reliability. APTW images almost showed the same compared with T1-weighted contrast-enhancing (T1W+C) images. The tumor rAPTW values had a strong positive correlation with pathologic grades.