Brown adipose tissue (BAT) is a target fat compartment for treatment of metabolic diseases due to its high metabolic capacity. BAT is major site for adaptive thermogenesis involving uncoupling protein-1. We have studied the dynamic oxidative fat metabolism in interscapular brown adipose tissue by activation of β3-adrenergic receptors. Progressive reduction of the lipids from iBAT region is indicative of oxidative metabolism by utilizing the lipids as fuel substrate. Evaluation of lipid mobilization in real time is important to assess the altered metabolic rate and mitochondrial biogenesis involving lipid oxidative metabolism.