The ACL-injured population is predisposed to developing radiographic Osteoarthritis post injury. Quantitative biomarkers such as T2 relaxation times evaluate the macromolecular composition of the cartilage matrix. Assessing the variation in this quantitative measure between knees of healthy individuals can help us identify degenerative difference brought on by injury. Using projection maps and pre-defined compartments, we measured the T2 relaxation times and quantified intrinsic differences between the knees of a group of healthy volunteers using coefficient of variation. This study demonstrates that there are similar T2 relaxation times for the left and right knees of healthy individuals.