This study aimed at identifying differences in T2 and T2* relaxation times in knee cartilage with and without the use of fat suppression. Four volunteers were scanned with 3D cones, 2D multi-echo FSE, and qDESS sequences with and without fat sat. For cones and qDESS, T2* and T2 values increased with use of fat sat. For 2D FSE, values did not change. The results show that use of fat sat can have an effect on quantitative MRI parameters.