FMRI has been widely used to assess changes in brain activity evoked by innocuous and noxious stimuli. However, stimulus-evoked fMRI (se-fMRI) measurements in mice have turned out challenging, and it is still under investigation whether and under which conditions se-fMRI applications in mice can lead to reliable readouts. Generally, se-fMRI could be a useful tool to characterize genetically modified mouse strains, such as mice exhibiting altered nociception. In this study, NaV1.7fl/fl:AdvCre mice were characterized with respect to neural processing of different types of peripheral stimuli and compared to a wildtype control group. Results of behavioral tests are compared to outcomes of fMRI and spectroscopic measurements.