We explored the radiographic features of isocitrate dehydrogenase (IDH) wildtype and mutated tumors in glioma patients preoperatively using APTW imaging at 3 Tesla. 26 patients with newly suspected low-grade gliomas were recruited, surgical specimens were obtained for pathological analysis, and corresponding APTW signal intensities were recorded. Results showed that the IDH1 wild-type tumors had significantly higher APTW signal intensity than the IDH1 mutant tumors. The area under the ROC curve (AUC) for APTW to differentiate IDH1 wild-type from IDH1 mutant was 0.750. APT imaging has the potential for discriminating IDH genotypes in WHO grade-II gliomas.