Most concussive brain injury is not readily detected in conventional MRI or standard DTI, requiring advanced imaging methodology. However, reliability of any newly developed imaging technology should be tested prior to starting full scale studies. Here we present a protocol which will enable inter-lab comparison of any advanced diffusion imaging technology with objective quantitative analysis. High spatial resolution diffusion imaging with optimized multiband pulse sequence performed on subjects twice at 1.25 mm isotropic voxel size. Post image processing including outlier exclusion and distortion correction was optimized. Brain parcellation based quantitative analyses was used to provide objective measures of reproducibility.