This study aimed to characterize changes in Neuromelanin-sensitive MR images with Parkinson’s Disease (PD) progression. The area of high signal intensity in the substantia nigra (SN) and its contrast ratio (CR) were assessed in early and late stage PD (LSPD) patients, and in healthy individuals. The relative hippocampus and midbrain volumes were also estimated from anatomical MPRAGE scans. The SN area was found to be markedly reduced in LSPD compared to early stages of the disease and so could be a useful tool to evaluate disease progression. The decrease in relative hippocampus volume could indicate cognitive impairment, characteristic of LSPD.