Quantitative assessment of carotid plaque burden is required to monitor the effects of treatments for carotid atherosclerosis. Cervical carotid plaque volume in 13 patients with carotid artery stenosis was measured and evaluated using manual and automated software methods for 3D T1 black-blood MRI. Measurement reproducibility was better using the automated method than that using the manual method. The automated software developed for the measurement of carotid plaque volume was feasible and reliable and could significantly reduce the measurement time. This method appears to be of great value in clinical settings.