Multiple-mouse MRI was implemented on a 7-Tesla magnet with four Cryogen (liquid helium)-cooled radio frequency probes. The high signal-to-noise ratio offered by the probes enabled spatial resolutions of 60 μm isotropic in <2 hours, a significant improvement for in vivo anatomical imaging of the mouse brain. Several 3D pulse sequences including FLASH/MP2RAGE/RARE were implemented for multiple-mouse acquisition. Manganese-enhanced T1-weighted images were obtained at two different resolutions of 60 μm and 75 μm. Results demonstrate the combined benefits of cryogen-cooled coils and multiple-mouse MRI.