This study aimed to evaluate sex and segment differences of myocardium in native T1 value and ECV in healthy volunteers. We measured native T1 value of 425 segments and ECV of 424 segments. The results showed that myocardial ECV of females was higher than that of males in basal, middle and apical segments. The ECV of the apical segments was higher than in the basal and middle segments. The mean native T1 value and ECV were not associated with age, ejection function(EF), end diastolic volume(EDV), end systolic volume(ESV), and stroke volume(SV).