Development of T1 mapping in the myocardium in recent years has demonstrated that the technique can add information to inform the diagnosis or management of patients in a number of pathologies. Sequences have been developed to enable T1 mapping of the myocardium within a single breath-hold and the limitations of these sequences have been well characterized. Recent studies have investigated myocardial T1 mapping in iron overload patients using variants of the MOLLI sequence. These studies have concluded that T1 mapping could be more sensitive and reproducible than T2* mapping for the measurement of iron overload in the myocardium. The current study aims to determine the optimal T1 mapping sequence for iron overload. Simulations, phantom studies and in-vivo studies were carried out to investigate MOLLI and SASHA sequences used for T1 mapping of low T1 and T2 tissues (comparable to iron overload in the myocardium).