MRI techniques probing brain perfusion and blood-brain barrier (BBB) permeability were assessed in their ability to detect low tumour invasion in mouse glioblastoma models. A multiple boli Arterial Spin Labeling technique was optimised, achieving high SNR perfusion imaging. Diffusion-weighted arterial spin labelling allowed to separate fast motion vascular components of the signal from slow motion tissue components, providing with BBB permeability weighted maps. Evaluation was performed by comparison with conventional MRI and immunohistochemistry sections (HLA) cut in the MRI plane . Both perfusion weighted maps and BBB permeability weighted maps allowed to identify low tumour regions not detected with conventional MRI techniques.