The purpose was to use intra-voxel incoherent motion (IVIM) measurement in DWI to distinguish benign from malignant breast tissues. IVIM-derived parameter maps D, f, and D* were computed using segmented fitting method with proper threshold b-value. The region of interests were avoided the area of calcification. With pathological diagnosis as the gold standard, receiver operating characteristic (ROC) curves for three parameters and f × D* reflect that the area under the curve (AUC) of D and f × D* are 0.862 and 0.726, respectively. It suggested that these two parameters have high significance for the diagnosis of breast cancer lesions.