This work monitored longitudinal changes in visceral (VAT) and subcutaneous (SCAT) adipose tissue in response to weight loss. Twenty-one patients undergoing clinical weight loss surgery (WLS) were recruited for 5 MRI studies: 2-3 weeks prior, 1 day prior to WLS and 1, 3, and 6 months post-WLS. Single breath-hold, chemical shift encoded acquisitions were analyzed using an Osirix semi-automated segmentation software to monitor changes in VAT and SCAT volumes. Continual reductions in VAT and SCAT were measured over the five visits. At every visit, each patient’s relative body fat distribution remained relatively constant despite overall reductions in both VAT and SCAT.