Bile exerts multiple functions in the liver and gut with a crucial role for triglyceride-, sterol- and carbohydrate-metabolism and is a key player in disease processes. The study purpose was to develop a reliable MRS protocol and to assess variability of bile acid determination in human gallbladder. Our study demonstrated higher stability and reliability of gallbladder spectra with subjects measured in prone position compared to back position. Relatively small coefficients of variation were obtained in a reproducibility study particularly within subjects, suggesting clinical applicability of the method, especially for longitudinal studies.
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