Imaging of drug delivery is useful for improving our understanding of the physical factors that contribute to the efficacy of anticancer therapies. Gemcitabine, a standard chemotherapeutic agent, has two hydroxyl groups and one amine group, making it potentially amenable to CEST imaging via proton exchange with tissue water. Recently, Li et al. (2016) showed that many anticancer drugs can induce CEST contrast and demonstrated that liposome-encapsulated gemcitabine can be imaged 5-hours post-administration in a pre-clinical model of cancer1. Here, we extend this exciting work and investigate the use of CEST to image acute gemcitabine uptake within 30 minutes of administration.