Colorectal cancer (CRC) is one of the most common malignant tumors in gastrointestinal tract. Tumor recurrence and metastasis are still a major cause of death in rectal cancer patients. Epithelial-mesenchymal transition (EMT) is reported a critical process providing tumor cells with the ability to migrate and metastasize to distant sites, leading to a poor prognosis in CRC. The diffusion kurtosis imaging (DKI) could quantify non-Gaussian behavior of water diffusion and provide more precise information of tissue characteristics. Our study demonstrated that DKI could be used to identify EMT in CRC xenograft models.