Colorectal cancer (CRC) is the third most common cancer in the world and its incidence is on the rise. Management is particularly challenging technically for surgeon and local recurrence is a common result of treatment failure. Tumor differentiation grade is one of the factors that influence the choice of individual management, then affecting the prognosis. Diffusion kurtosis imaging (DKI) could quantify non-Gaussian behavior of water diffusion and provide more precise information of tissue characteristics. Our study demonstrated that DKI is conducive to distinguishing the various differentiations of CRC models, and reflecting the proliferation indirectly in tumor cells.